High throughput analysis of <scp>MHC?I</scp> and <scp>MHC?DR</scp> diversity of Brazilian cattle populations

The major histocompatibility complex (MHC) contains many genes that play key roles in initiating and regulating immune responses. This includes the polymorphic MHCI MHCII present epitopes to CD8+ CD4+ T-cells, respectively. Consequently, characterisation of repertoire MHC is an important component improving our understanding genetic variation determines outcomes In cattle, (BoLA) research has predominantly focused on Holstein-Friesian animals (as most economically breed globally), although development high-throughput approaches allowed BoLA-DRB3 be studied a greater variety breeds. previous study we reported MiSeq-based method enable high-resolution analysis bovine repertoires. Herein, report expansion this methodology incorporate its application analyse diversity large cohort cattle from Brazil (>500 animals), including representatives three Bos indicus breeds – Guzerat, Gir Nelore. large-scale description paired MHCI-DRB3 repertoires identified small number novel DRB3 alleles, alleles haplotypes, provided insights into MHCI-MHCII association - further expanding knowledge diversity.